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| - | {{ : | + | ====== Cryopreservation and In Vitro Fertilization (IVF) Protocols |
| - | ====== Cryopreservation and In Vitro Fertilization (IVF) Protocol | + | |
| - | * ZIRC E400/RMMB Sperm Cryopreservation & IVF Protocol | + | * {{ : |
| * Complete description of ZIRC's current cryopreservation & IVF protocols | * Complete description of ZIRC's current cryopreservation & IVF protocols | ||
| - | * Step-by-Step Cryopreservation: | + | * [[: |
| * Abridged version of ZIRC's cryopreservation & IVF protocols | * Abridged version of ZIRC's cryopreservation & IVF protocols | ||
| - | * ZIRC NanoDrop 2000 Calibration Curve and Sperm Density Calculator | + | * {{ : |
| * ZIRC's NanoDrop 2000 hemocytometer-generated standard curve and sperm concentration calculator for use with OD400 measurements for stripped sperm in E400 extender | * ZIRC's NanoDrop 2000 hemocytometer-generated standard curve and sperm concentration calculator for use with OD400 measurements for stripped sperm in E400 extender | ||
| - | * Diluting Pooled Sperm based on Nano Drop Cell Counts | + | * {{ : |
| * Detailed description of how to use a NanoDrop 2000 spectrophotometer for measuring sperm cell concentrations and how to use the Excel Pooled Sperm Dilution Worksheet | * Detailed description of how to use a NanoDrop 2000 spectrophotometer for measuring sperm cell concentrations and how to use the Excel Pooled Sperm Dilution Worksheet | ||
| - | * Pooled Sperm Dilution Worksheet | + | * {{ : |
| - | * A blank Excel sperm dilution worksheet for use with Nanodrop 2000 cell counts as described in Diluting Pooled Sperm based on NanoDrop Cell Counts | + | * A blank Excel sperm dilution worksheet for use with Nanodrop 2000 cell counts as described in Diluting Pooled Sperm based on NanoDrop Cell Counts |
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| - | * Step-by-Step Cryopreservation: | ||
| - | * [[step-by-step_cryopreservation_protocol]] | ||
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